What is it about?

Methodology and calibration of new analytical techniques, the GreenLight™ system for rapid enumeration of total viable counts (TVC) was introduced. The system is based on the principle of quenching of luminescence intensity and lifetime of an oxygen-sensitive dye by sample O2 consumed during sample incubation and growth of microflora. The new detection methodology was compared with agar plating EN ISO 4833:2003 method showing excellent correlation. After calibration, the system enables to determine the numbers of microorganisms within 24 hours.

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Why is it important?

As the determination is based on detection of oxygen consumption by live active cells it enables fast determination of viable microorganisms in foods. The higher microbial load is in the food sample the shorter time for determination of viable count is needed. Thus, in case of simple food matrix, the results can be reached even within one shift of production.


Promising technique not only for fast detection of microbial number in foods, but also for indication of viable cells applicable, e.g. in non-sterility detection, contamination/recontamination, efficacy of cleaning and disinfection, etc.

prof. Ľubomír Valík
Slovak University of Technology in Bratislava

Read the Original

This page is a summary of: Application of a new method to control microbial quality of foods based on the detection of oxygen consumption, Acta Chimica Slovaca, January 2016, De Gruyter,
DOI: 10.1515/acs-2016-0004.
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