Tannins analysis from different medicinal plants extracts using MALDI-TOF and MEKC

Alina Oana Matei, Florentina Gatea, Eugenia Dumitra Teodor, Gabriel Lucian Radu
  • Chemical Papers, January 2016, Springer Science + Business Media
  • DOI: 10.1515/chempap-2015-0222

Tannins analysis from different medicinal plants

What is it about?

The matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF) and micellar electrokinetic chromatography (MEKC) methods were used to identify and quantify five tannins, (+)-catechin, (-)-epigallocatechin, (-)-epigallocatechin gallate, (-)-epicatechin gallate and (-)-epicatechin, from aqueous, ethanolic and acetonic extracts of Calendula officinalis, Hypericum, perforatum, Galium verum and Origanum vulgare. The MALDI-TOF technique was used for screening tannins monomers and oligomers in plant extracts. The sandwich method and matrix 2,5-dihydroxybenzoic acid with a concentration of 10 mg mL -1 in acetonitrile/ultrapure water/trifluoroacetic acid (20 : 80 : 0.1, vol.) were used. The electrophoretic method developed for the separation and quantification of 5 catechins in 15 min exhibited good efficiency and precision, low limits of detection (0.0032-0.0153 μg mL -1 ) and quantification (0.0096-0.0466 μg mL -1 ). The correlation coefficients (R 2 ) exceeded 0.9986 and the recovery values ranged between 94.25 % and 102.50 %. The present work provides new information on some of the less studied compounds present in plants frequently used in traditional medicine.

The following have contributed to this page: Dr Eugenia Dumitra Teodor and Prof.dr.eng Gabriel Lucian RADU