Analytical aspects of the antinuclear antibody test by HEp-2 indirect immunofluorescence: EFLM report on an international survey

What is it about?

Detection of antinuclear antibodies (ANA) by indirect immunofluorescence assay using HEp-2 cells (HEp-2 IFA) is used to screen for various autoimmune diseases. We report on a questionnaire that was developed to collect information on Hep-2 IFA methodology, computer-assisted diagnosis systems, training, inter-observer variability, quality assessment, reagent lot change control, and method verification.

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