Sensitivity of the Sysmex XN9000 WPC-channel for detection of monoclonal B-cell populations

  • Margreet Schoorl, Marianne Schoorl, Monique Chevallier, Jeanette Oomes, Johannes van Pelt
  • Clinical Chemistry and Laboratory Medicine (CCLM), January 2017, De Gruyter
  • DOI: 10.1515/cclm-2016-1075

WPC performance on Sysmex XN systems

What is it about?

With the introduction of the Sysmex XN Haematology analyser the white blood cell differential channels (WNR and WDF) and abnormal cell detection channel (WPC) have been introduced for flagging blasts and abnormal lymphocytes. In this study the performance characteristics of the WBC suspect flags were compared with immunophenotyping results.

Why is it important?

After immunophenotyping subjects were classified as cases with B-ALL, B-CLL, B-NHL, B-NHL/Hairy Cell Variant, MBL and AML according to the WHO criteria for immunophenotyping (2008). Our study resulted in 30 true-positive cases, 6 truenegative cases, 2 false-positive cases and 17 false-negative cases. A MBL was demonstrated in 12 out of the 17 false-negative cases.

Perspectives

Margreet Schoorl
Northwest Clinics (formerly named: Medical Center Alkmaar)

To conclude, with XN software version 00.15, the performance of the WPC reflex flag for the detection of small monoclonal B-cell populations is inappropriate. In order to improve the sensitivity of the WPC flagging for the detection of small monoclonal B-cell populations, we recommend to include the absolute lymphocyte count within the XN flagging strategy.

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http://dx.doi.org/10.1515/cclm-2016-1075

The following have contributed to this page: Margreet Schoorl