Cytological evaluation of pleural effusion with cell block techniquein a dog

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In this case, the use of the Cell Block technique in the diagnosis of pleural effusion collected from a 3.5 year old etter Spaniel breed dog was investigated. The dog was presented to Ankara University Small Animal Hospital with the history ofacute dyspnea, weakness, exercise intolerance and tachypnea for a week. Routine clinical examinations including blood testing wereperformed. Abdominal distension, pleural effusion and cyanotic cranial mucous membranes were remarkable during physicalexamination. The pleural fluid collected by thoracocentesis was brownish-colored, cloudy and has contained granular materials. The specimen collected from the dog was separated into three aliquots, one part was processed in a routine manner for cytological xamination by cytocentrifugation, and the other parts of the sample were used for a cell block and bacteriological analysis. Inmicrobiological analysis, Escherichia coli and Klebsiella spp. were isolated. The cytocentrifuged preparations and cell block sections evealed septic effusion. The cell block technique demonstrated morphological details better than cytocentrifugation. In conclusion, in this case, cytology and bacteriology of effusion, routine blood tests, abdominal radiography and echocardiography showed that thepleural effusion was resulted from pyothorax due to severe tricuspid dysplasia.

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