What is it about?
In current study, we focused on methodology of high-impact cell culture papers to evaluate adequacy of the evidence they provided to support their claims. We identified common claims put forward (such as changes in viability, cytotoxicity, proliferation/growth rate and cell death) and investigated if the experimental design is appropriate to measure related claims. We detected multiple cases of misinterpretation of assay results, inappropriate (or completely lacking) evidence for claims, missing information and other issues.
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Why is it important?
There is an alarming quality crisis in cell culture studies. Confusion regarding the terminology and misinterpretation of assays are amongst the causes. Here we report that even high-impact studies fail to justifiably interpret cell culture methods. We also provide clear and distinct definitions for each term (viability, cytotoxicity, proliferation/growth rate and cell death) and discuss best ways to measure them.
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This page is a summary of: From viability to cell death: Claims with insufficient evidence in high-impact cell culture studies, PLoS ONE, February 2022, PLOS, DOI: 10.1371/journal.pone.0250754.
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