What is it about?
An holographic microscopy setup using two illumination beams is proposed to image blood microcirculation in preclinical models. Greedy algorithm reconstruction of the two resulting holograms leads to a 3D reconstruction of the sample over time.
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Why is it important?
We proposed a technique that combines digital holography, dual illumination of the sample and reconstruction with a greedy algorithm to obtain 4D images of blood microcirculation in a living zebrafish embryo.
Perspectives
This technique can be also applied to other biological samples that needs 3D visualization over time. The possibility to check the recostruction quality in real time and the rapid processing of the data are fundamentals skill of this technique.
Dario Donnarumma
Laboratoire Charles Coulomb
Read the Original
This page is a summary of: 4D holographic microscopy of zebrafish larvae microcirculation, Optics Express, November 2016, Optical Society of America (OSA),
DOI: 10.1364/oe.24.026887.
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Resources
Visualization 1
Reconstructed intensity image of RBCs made with the 2-phase hologram, z = 0
Visualization 2
Reconstructed intensity image of RBCs made with the 2-phase hologram, z = 26.7 μm
Visualization 3
Reconstructed intensity image of RBCs made with the 2-phase hologram, z = 53.5 μm
Visualization 4
Averaged intensity reconstructed images made with 2 phases holograms
Visualization 5
3D reconstruction made without the cleaning algorithm.
Visualization 6
3D reconstruction made with the cleaning algorithm.
Visualization 7
Final 4D reconstruction
Contributors
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