An Immunohistochemical Study of N-Methyl-D-Aspartate Receptors in Human Cerebellum and Hippocampus

Euphrosyni S. Koutsouraki, John J. Anastasiades, Stavros J. Baloyannis
  • American Journal of Medical Sciences and Medicine, April 2012, Science and Education Publishing Co., Ltd.
  • DOI: 10.12691/ajmsm-1-2-3

An Immunohistochemical Study Receptors in Human Cerebellum and Hippocampus

What is it about?

The aim of our investigation was to demonstrate the wide distribution of glutamate N-methyl-D-aspartate receptors (NMDA R), particularly the not-widely investigated 2A and 2B subunits, in the human adult cerebellum and hippocampus. Specimens of human hippocampus and cerebellum were obtained at the time of autopsy from two male individuals, aged 24 and 48 years, with no obvious brain injury. The brains were immunostained using anti-human rabbit polyclonal NR antibodies (NMDA R 2A&B, ΑΒ1548 (Chemicon), NMDA receptors anti- human Poly HRP IHC (Detection kit) and counterstained with Mayer’s hematoxylin. The present immunohistochemical research of human adult cerebellum and hippocampus demonstrates that the majority of neurons in the dentate gyrus, the large pyramidal neurons of the hippocampus, the granular cells of the cerebellum as well as the main cerebellar neuron, namely Purkinje cell, stained deeply by the monoclonal antibody, suggesting that the majority of the neuronal network in cerebellum and hippocampus uses as neurotransmitter the excitatory amino acids on the system of NMDA receptors. Our findings, demonstrating that the majority of cells were stained by the monoclonal antibody, emphasize the importance of the excitatory system of the glutamate and the examined receptors 2A & B, in the human cerebellum and hippocampus in adults, underlying the important role that this system may play in memory function and cognition, and at the same time the crucial role of the cerebellum in higher cognitive functions, in part due to the wide distribution of NMDA R.

Why is it important?

The present immunohistochemical research of human adult cerebellum and hippocampus, demonstrates that the majority of neurons in the dentate gyrus, the large pyramidal neurons of the hippocampus, the granular cells of the cerebellum as well as the main cerebellar neuron, namely Purkinje cell, stained deeply by the monoclonal antibody, uses as neurotransmitter the excitatory amino acids, on the system of NMDA R 2A&B. Since it isn’t well known the distribution of NMDA R, especially the subtypes 2A&B, in human hippocampus and cerebellum in adults, our findings emphasize the importance of the excitatory system of the glutamate in these areas, underlying the important role that this system may play in memory function and cognition and at the same time the crucial role of the cerebellum in higher cognitive functions, in part due to the wide distribution of NMDA R. References

Perspectives

Professor Stavros J Baloyannis (Author)
Aristotle University of Thessaloniki

NMDA-Rs increase the depolarization induced by the complex spike and prolong its duration. These effects are expected to favor calcium entry in the dendrites as well as their propagation. Thus, NMDA-Rs are likely to be players in the game of mature Purkinje cells plasticity

Read Publication

http://dx.doi.org/10.12691/ajmsm-1-2-3

The following have contributed to this page: Professor Stavros J Baloyannis