What is it about?
A cultured plug of E. coli was sent to four separate labs, processed for sequencing using a standardised sample preparation procedure, then sequenced over two 48 hour runs by each of the four labs. A brief analysis was carried out on the sequence data, investigating a number of different statistics including error rate, yield over time, and the composition of sequenced reads.
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Why is it important?
This is the first publication from the MinION Analysis and Reference Consortium, a global and open group of MinION researchers who are investigating the behaviour and output of the MinION technology platform. As well as providing a benchmark for subsequent nanopore flow cell technology, this paper provides a large dataset of a known genomic sequence for further research and discovery.
Perspectives
The MARC team is fairly bioinformatician-heavy, so my usual/preferred skills in data analysis are not in high demand. I have been taking extensive notes during the regular MARC teleconferences, and provide the occasional comment when I think something needs more clarification or could be misinterpreted. As I did not take part in the actual sequencing or data analysis, my specific contribution to this paper was minimal: mostly around copy editing to make the paper a little bit easier to understand.
Dr David A Eccles
Malaghan Institute of Medical Research
Read the Original
This page is a summary of: MinION Analysis and Reference Consortium: Phase 1 data release and analysis, F1000Research, October 2015, Faculty of 1000, Ltd.,
DOI: 10.12688/f1000research.7201.1.
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