Positively sorted cells using Antibody conjugated magnetic microbeads have impaired functionality

What is it about?

Positively sorted CD14+ cells using antibody conjugated paramagnetic microbeads exhibit hampered functionality and may result in inaccurate analysis and observations in downstream applications. These cells can be used for immediate analytical procedures.

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Why is it important?

Magnetic sorting is a common technique used to obtain a highly pure population of cells of interest from a mixed population of cells. Positive sorting involves the tagging of cells with magnetic microbead conjugated antibodies, followed by isolation of the labeled cells by placing them in a magnetic field. After positive sorting, cells that have microbead conjugated antibodies on their surface can be conveniently analyzed using flowcytometry. Positively sorted cells have impaired functionality and may result in inaccurate analysis and observations in downstream culture and applications.