What is it about?
We showed that when confocal image signals were very weak, the commercial Huygens image deconvolution software removed meaningful data. A workaround employing a standard image filter preserved the data. In collaboration with the company that makes Huygens, we subsequently confirmed that an updated version of the software fixed the problem.
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Why is it important?
Huygens is used routinely in many laboratories. By identifying and overcoming a limitation in the algorithm, we improved the sensitivity and reliability of image deconvolution for a large number of researchers.
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This page is a summary of: Improved deconvolution of very weak confocal signals, F1000Research, June 2017, Faculty of 1000, Ltd.,
DOI: 10.12688/f1000research.11773.1.
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