What is it about?
We demonstrate how to organize bacterial pelleting, resuspension, and mixing of solutions for cell lysis and plasmid DNA purification to obtain high-quality plasmid DNA. We demonstrate how the mixing mode of solutions can influence the stable structure of plasmid DNA. In addition to the ease of plasmid isolation using this device, it saves up to 70% of the time required to process 12 samples compared to the standard miniprep method.
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Why is it important?
Using the device, we expanded the scope of application of miniprep plasmids and showed that the quality of the plasmids is sufficient for their use in protein/peptide-DNA binding studies, and plasmids purified using this device can be widely used in DNA methylation analysis.
Perspectives
Due to the extreme simplicity of plasmid DNA isolation and the reduction in plasmid isolation time, the use of the device eliminates unnecessary labor and will contribute to progress in molecular biology, where plasmids are widely used.
Mikhail Baryshev
Read the Original
This page is a summary of: A new device-mediated miniprep method, AMB Express, February 2022, Springer Science + Business Media,
DOI: 10.1186/s13568-022-01360-7.
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