What is it about?
The report concerns the composition of the bacterial community in the gut of peridomestic triatomines characterized by sequencing the cytochrome c oxidase subunit I (COI) gene. The bacterial community was investigated using culture independent methods based on the amplification of the 16S rRNA gene by PCR, denaturing gradient gel electrophoresis (DGGE), DNA fragment cloning, Sanger sequencing and 454 pyrosequencing. Additionally, we identified TcI and TcII types of T. cruzi by sequencing amplicons from the gut metagenomic DNA with primers for the mini-exon gene.
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Why is it important?
Triatomines from the genus Triatoma are frequently collected in the peridomestic ecotopes where they interact with dogs and humans. Trypanosomiasis is still a deleterious disease for which no treatment is available yet. Thus, the best route to control it is still its prevention. Our report shows that Serratia is the main bacterial genus present in the microbiota from the digestive tract of triatomine where T. cruzi thrives as well since it was encountered in all samples analyzed by DGGE and 454 pyrosequencing. For this reason, Serratia could be contemplated for para-transgenesis.
Perspectives
The para-transgeneis of Serratia for synthesis of microbial peptides should be tested for the epidemiological control of T. cruzi through the inhibition of its proliferation within triatomines.
Nicolas Carels
Oswaldo Cruz Foundation
Read the Original
This page is a summary of: Characterization of the microbiota in the guts of Triatoma brasiliensis and Triatoma pseudomaculata infected by Trypanosoma cruzi in natural conditions using culture independent methods, Parasites & Vectors, April 2015, Springer Science + Business Media,
DOI: 10.1186/s13071-015-0836-z.
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