What is it about?
The reports describes a large-scale screening of potential candidate reference genes from RNA-seq data as well as their validation by qRT-PCR, and careful examination of reference data from the International Cancer Genome Consortium, The Cancer Genome Atlas and Gene Expression Omnibus repositories.
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Why is it important?
The method described highlights the importance of a massive evaluation of housekeeping genes to assess their relevance as internal control for optimized intra- and inter-assay comparison of gene expression. Taking an example, many control genes such as actin genes are not suitable to assess malignant tissues because the expression of these genes can be up-regulated, especially in the metastaic state.
Perspectives
The strategy described for the identification of housekeeping genes as internal control for the intra- and inter-assay comparison of gene expression in breast cancer can be applied to other tumor types and diseases.
Nicolas Carels
Oswaldo Cruz Foundation
Read the Original
This page is a summary of: A strategy to identify housekeeping genes suitable for analysis in breast cancer diseases, BMC Genomics, August 2016, Springer Science + Business Media,
DOI: 10.1186/s12864-016-2946-1.
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