What is it about?
We evaluated statistically the influence of several parameters on PCR efficiency (i.e. amplicon length, primers GC content, primer dimers, etc) and implemented a Web tool to aid primer design while maximizing the predicted PCR efficiency.
Featured Image
Why is it important?
The Web tool is freely available at http://srvgen.upct.es/efficiency.html
Read the Original
This page is a summary of: pcrEfficiency: a Web tool for PCR amplification efficiency prediction, BMC Bioinformatics, January 2011, Springer Science + Business Media,
DOI: 10.1186/1471-2105-12-404.
You can read the full text:
Contributors
The following have contributed to this page
