What is it about?

Fibrosis is a devastating diseases and animal model systems that adequately resemble diseases pathology are missing. Here we show how we can use ex-vivo cultured live human lung tissues (hPCLS) to study lung diseases, in particular, interstitial lung diseases. The study has highlighted what are the factors that should be considered while studying fibrosis signalling in hPCLS model system.

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Why is it important?

Our findings reveal that lung tissue derived from tumour free areas of lung resections, show intracellular activation of pro-fibrotic signalling upon TGFbeta1 treatment but at the same time modulates metalloproteinase expression to counterbalance deposition of collagen in extracellular matrix.


We hope that this study shines light that the deposition of fibrillar collagens in ECM is human lung tissue is a complex process and indirect measurement are not adequate to claim that activation of intracellular pro-fibrotic signalling will result in excessive deposition of ECM.

Muzamil Majid Khan
European Molecular Biology Laboratory

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This page is a summary of: An integrated multiomic and quantitative label-free microscopy-based approach to study pro-fibrotic signalling in ex vivo human precision-cut lung slices, European Respiratory Journal, December 2020, European Respiratory Society (ERS), DOI: 10.1183/13993003.00221-2020.
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