100.414 kHz EMF enhance inducing capacity of LCA and 10% PBS Washouts of Different Holocene Minerals

What is it about?

Macrophages play key role in host defense and tissue repair, and thus understanding regulation of their function is important. For instance, our previous results have shown that in chicken macrophage system (CoMA cell line), application of a pulse of electromagnetic fields of frequencies 0.618, 1.054, 5.229, and 100.414 kHz induces production of interferon γ-like molecules. In this study, we have shown that the electromagnetic field of 100.414 kHz is the most effective in inducing synthesis of chicken interferon γ and chicken interferon γ-like molecules in CoMA cells, especially when combined with Lens culinaris agglutinin and 10% phosphate-buffered saline washouts of different Holocene minerals. A 2-minute pulse of electromagnetic field was produced by Defender’s pulse generator. Both chicken interferon γ and chicken interferon γ-like molecules from the cell supernatant were evaluated by an antiviral assay and were also analyzed with reverse-phase high-performance liquid chromatography on Phenomenex, Aeris peptide columns. Our results show that application of a single inducing factor (Lens culinaris agglutinin, 100.414 kHz electromagnetic field, 10% phosphate buffer saline washout) or combined usage of 2 of them moderately stimulated production of chicken interferon γ-like molecules (from 1.550 to 48.028 IU/mL), whereas the combination of 10% phosphate-buffered saline washout of Koprivnica rock + Lens culinaris agglutinin + 100.414 kHz/9 V resulted in an output of 162.122 IU/mL. Hence, we may conclude that a combined use of electromagnetic field, Holocene minerals, and Lens culinaris agglutinin greatly stimulates synthesis of chicken interferon γ-like molecules in CoMA cells. Keywords CoMA cells, ChIFN γ, LCA, 10% PBS washout, Holocene minerals, induction, antiviral assay, RP-HPLC

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Why is it important?

In conclusion, 10%PBS washouts of different Holocene min-erals (Table 3) enhanced the LCA induction of ChIFNg-likemolecules synthesis with the use of EMF of 100.414 kHz/9 Vfor 2 minutes. Sand from Koprivnica (sample 3) in a combina-tion of 10%PBS washout of sample 3þLCAþ100.414 kHz/9V for 2 minutes gave 162.122 (409) IU/mL. Therefore it is possible that results depend on the specific chemical composi-tion of various Holocene minerals. It is also important to get the cheap way of production of ChIFNgand ChIFNg-like molecules because of their use for poultry infection diseases therapy, as well as for cancer therapy in combination with the NDVvirus ZG1999HDS.

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