What is it about?
We report a highly rapid, visual, precise, selective and sensitive analytical method for the determination of hydrogen peroxide (H2O2 in milk using Graphene oxide (GO) with 2,7-dichlorfluorescein diacetate (DCFH-DA). A 1000 L aliquots of 10-fold diluted samples (high and low-fat milk) directly onto the 100 L of GO and 100 L of 100 M DCFH-DA produced green colour under Ultraviolet light at 365 nm. The analytical feature of our proposed method includes low detection limit (10 mmol mL−1 and satisfactory recovery values for samples.
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Why is it important?
We report a highly rapid, visual, precise, selective and sensitive analytical method for the determination of hydrogen peroxide (H2O2 in milk using Graphene oxide (GO) with 2,7-dichlorfluorescein diacetate (DCFH-DA). A 1000 L aliquots of 10-fold diluted samples (high and low-fat milk) directly onto the 100 L of GO and 100 L of 100 M DCFH-DA produced green colour under Ultraviolet light at 365 nm. The analytical feature of our proposed method includes low detection limit (10 mmol mL−1 and satisfactory recovery values for samples. The presence of H2O2 in milk is a major concern because it constitutes a public health hazard. Many milk indursties are using H2O2 as a preservative, but if the concentration increases then it causes so many health problems such as neurodegenerative disorders, cancer and diabetes. Present methods show an easy way for detecting H2O2 generally require considerable time and laboratory facilities. The chemical tests have sufficient sensitivity to detect wide linear range of H2O2 concentration.
Perspectives
Photoluminescence is one of the most popular methods of detection in analytical chemistry because of its high sensitivity. Graphene oxide has shown unique optical properties due to its large surface to volume ratio and unique electronic structure that has aided in the PL detection of H2O2 in milk. Here in we have successfully demonstrated a sensitive, accurate, low cost and rapid spectrophotometric method for H2O2 determination based on GO. The proposed method has been satisfactorily used for quantitative determination of H2O2 levels in milk with low detection limit and over a wide concentration range. Most of the currently available methods for H2O2 detection in milk focus on the lower limit, but in low concentration H2O2 is used as a preservative. In contrast, our research provides a clear platform for the detection and quantification of high concentrations of H2O2 in milk. It could be envisaged that detection sensitivity could be improved through further experimental condition optimization and integrating with microfluidic devices to realize H2O2 detection in each event. Although detection with the naked eye seems perfectly suited for detecting H2O2, it is inherently difficult to quantify the concentration. Nevertheless, this potential limitation cannot stop it from becoming a promising and versatile toolkit for signal amplification in the detection of H2O2 using the naked eye.
Dr. Sitansu Sekhar Nanda
Myongji University
Read the Original
This page is a summary of: Graphene Oxide Based Fluorometric Detection of Hydrogen Peroxide in Milk, Journal of Nanoscience and Nanotechnology, January 2016, American Scientific Publishers,
DOI: 10.1166/jnn.2016.10646.
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