Withania Somnifera (Ashwagandha) Root Extract Using LC-MS, GC-MS, and NMR Spectroscopy

What is it about?

The current study was designed to investigate the impact of Consciousness Energy Healing Treatment (The Trivedi Effect®) on the characteristic properties of the phytoconstituents present in the ashwagandha root extract using LC-MS, GC-MS, and NMR spectroscopy.

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Why is it important?

The current study infers that Consciousness Energy Healing Treatment (The Trivedi Effect®) has the significant effect on the peak area% i.e. the relative concentration as well as the peak intensities of the phytoconstituents in ashwagandha root extract without affecting their structural properties. The LC-MS analysis revealed that the retention time of the phytoconstituents remained same in both the control and treated samples, whereas the peak area% at respective retention time was significantly altered. The peak area% of the treated sample at Rt of 5.06, 5.24, 5.49, 5.68, 6.78, 6.87, 8.12, 8.22, 8.36, 8.55, 9.02, and 10.13 minutes were significantly increased in the range of 0.27% to 146.94% compared to the control sample. In addition, the peak area% of the treated sample at Rt of 5.29, 5.36, 6.40, 6.49, 6.56, 6.67, 7.08, 7.37, 7.76, 7.87, 8.02, 8.43, 8.85, 8.97, and 9.12 minutes were significantly decreased in the range of 1.83% to 23.53% compared with the control sample. A total of 18 withanolides such as sitoindoside IX, viscosa lactone B, 24, 25-dihydrowithanolide D, withanolide A, withaferin A, withanone, withanolide D, ixocarpalactone A, withanolide S, withanolide sulfoxide, etc. were proposed with their structure from the molecular mass at m/z 650, 489, 473, 471, 505, and 992 at retention times of 6.87, 7.10, 7.87, 8.22, 8.54, and 9.11 minutes with the help of GC-MS and NMR data of both the control and Biofield Energy Treated samples. The mass peak intensity values of at the same retention time in the Biofield Energy Treated sample was significantly decreased in the range of 3.99% to 52.28% compared with the control sample. The Energy of Consciousness Healing Treatment could be valuable for altering the concentration of the phytoconstituents in the ashwagandha root extract by modifying their intrinsic physicochemical properties, which might be helpful to improve the bioavailability of active constituents present in ashwagandha root extract that might provide better therapeutic response against various diseases various diseases such as allergies, diabetes mellitus, and septic shock; stress-related disorders like sleep disorder, anxiety, insomnia, Attention Deficit Disorder (ADD), depression, Attention Deficit Hyperactive Disorder (ADHD), brain frog, low libido, impotency, mental restlessness (mind chattering), lack of motivation, fear of the future, confusion, migraines, mood swings, headaches, forgetfulness, overwhelm, worthlessness, indecisiveness, loneliness, frustration, irritability, chronic fatigue, obsessive/compulsive behavior and panic attacks; inflammatory diseases and immunological disorders like Lupus, Hashimoto Thyroiditis, Systemic Lupus Erythematosus, Type 1 Diabetes, Asthma, Tuberculosis, Hepatitis, Chronic peptic ulcers, Chronic active hepatitis, Celiac Disease (gluten-sensitive enteropathy), Crohn’s disease, Graves’ Disease, Addison Disease, Pernicious and Aplastic Anemia, Irritable Bowel Syndrome (IBS), Sjogren Syndrome, Multiple Sclerosis, Rheumatoid arthritis, Ulcerative colitis, Chronic periodontitis, Chronic sinusitis, Myasthenia Gravis, Diverticulitis, Atherosclerosis,Vasculitis, Dermatitis, Rheumatoid Arthritis, Alopecia Areata, Psoriasis, Scleroderma, Reactive Arthritis, Fibromyalgia, Chronic Fatigue Syndrome and Vitiligo; aging-related diseases like cardiovascular disease, arthritis, cancer, dementia, cataracts, osteoporosis, Alzheimer’s disease, diabetes, hypertension, glaucoma, Parkinson’s Disease, Huntington’s Disease, hearing loss, Prion Disease, Spinocerebellar Ataxia,, Motor Neuron Disease, Amyotrophic lateral sclerosis, Spinal muscular atrophy, Friedreich’s Ataxia and Lewy Body Disease, chronic infections and much more.