Isotopic Abundance of 13C, 2H, or 15N in Biofield Energy Treated Aminopyridine Derivatives

What is it about?

2-Aminopyridine (2-AP) and 2,6-diaminopyridine (2,6-DAP) are two derivatives of aminopyridines that act as an important organic intermediates, mostly used in medicines, dyes and organic sensors. The aim of the study was to evaluate the impact of biofield energy treatment on isotopic abundance ratios of 2H/1H, 13C/12C, or 15N/14N, in aminopyridine derivatives using gas chromatography-mass spectrometry (GC-MS). The 2-AP and 2,6-DAP samples were divided into two parts: control and treated. The control sample remained as untreated, while the treated sample was further divided into four groups as T1, T2, T3, and T4. The treated group was subjected to Mr. Trivedi’s biofield energy treatment. The GC-MS spectra of 2-AP and 2,6-DAP showed five and six m/z peaks respectively due to the molecular ion peak and fragmented peaks of aminopyridine derivatives. The isotopic abundance ratio of 2H/1H, 13C/12C, or 15N/14N were calculated for both the derivatives and significant alteration was found in the treated samples as compared to the respective control. The isotopic abundance ratio of 2H/1H, 13C/12C, or 15N/14N in treated samples of 2-AP was decreased by 55.83% in T1 and significantly increased by 202.26% in T4. However, in case of 2,6-DAP, the isotopic abundance ratio of 2H/1H, 13C/12C, and 15N/14N, in the treated sample showed a significant increase (up to 370.54% in T3) with respect to the control. GC-MS data suggested that the biofield energy treatment on aminopyridine derivatives had significantly altered the isotopic abundance of 2H, 13C, or 15N in the treated 2-AP and 2,6-DAP as compared to the control.

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Why is it important?

Most of the elements occurred in nature as a mixture of isotopes. The relative abundances of isotopes is different at different places on the earth and remained constant for years. The distribution of contaminant sources of any molecule on a native or global scale can be understood by determining the isotopic abundance ratio [1]. Any kinetic process that leads to the local depletion or enhancement of isotopes in organic molecules can be successfully determined using gas chromatography-mass spectrometry (GC-MS) [2]. These deviations from perfect chemical equivalence are termed as isotope effects. The isotopic abundance ratio is commonly reported in terms of atom percent and determined by high resolution mass spectrometry (HRMS spectrometry) [3]. For example, 13C Moreover, the rate of chemical reaction may vary with the mass of the nucleus with different isotopic substitutions, which slightly affect the partitioning of energy within the molecules [4]. The aminopyridines, selected for this study are heterocyclic pharmacophores for many bioactive small organic molecules [5]. The growing interest of these molecules pharmacologically due to its rare tendency to be oxidized like aniline derivatives [6]. Aminopyridines avoid such problems due to its reduced oxidation potential, thus make it a safer alternatives for drug design. Additionally, they are advantageous because they have exhibited strong absorption and emission spectra and thus useful as fluorescent tags while investigating the activities on a target [7]. Aminopyridines are used as a drug for symptomatic treatment of multiple sclerosis by blocking potassium channels and prolonging action potentials [8, 9]. It has been used as intermediate for the synthesis of pharmaceutical agents such as piroxicam sulfapyridine, tenoxicam, and tripelennamine [6, 10]. Besides, diaminopyridines are mostly used for synthesis of dyes, cosmetics, drugs and explosives [11, 12]. It is also used as intermediate for the synthesis of epoxy curing agents, polyamides, and preparation of analgesic phenazo- pyridine hydrochloride [13]. Additionally both the aminopyridine derivatives were successfully utilized as organic fluorescence sensors for the detection of metal cation [14]. The isotopic abundances of ratios of 2H/1H, 13C/12C, or 15N/14N, could be locally altered by kinetically driven chemical reactions. There is an alternative and well-known approach, Mr. Trivedi’s biofield energy treatment also known as The Trivedi Effect®, that can be applied on aminopyridine derivatives to undergo the isotopic changes. The physicochemical properties of various molecules and crystals were already altered by utilizing Mr. Trivedi’s biofield energy treatment [15-17]. The National Center for Complementary and Alternative Medicine (NCCAM) has recommended the use of energy therapy as a part of Complementary and alternative medical therapies (CAM) in the healthcare sector [18]. CAM includes numerous energy-healing therapies, in which the biofield therapy is a form of putative energy therapy that is being widely used worldwide to improve the overall health of human beings. Humans have the ability to harness energy from the environment/universe that can be transmitted to any objects around. Based on the previous results achieved by the biofield energy treatments by Mr. Trivedi in various fields, biofield energy treated 2-AP and 2,6-DAP were taken for mass spectroscopy studies to evaluate the isotopic abundance ratio of 2H/1H, 13C/12C, or 15N/14N (PM+1)/PM, [where PM is the primary molecule and (PM+1) is isotopic molecule].

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