An in Vitro Study of Biofield Energy Healing Based Herbomineral Formulation for Skin Protection

What is it about?

The current research work deals with the impact of the Consciousness Energy Healing Treatment (The Trivedi Effect®) based herbomineral test formulation and cell medium (DMEM) against various skin health parameters.

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Why is it important?

In the present study, cell viability was significantly improved with viability in more than 75% cells in all the tested concentrations of the test formulation such as HFF-1 (1.25 to 10 μg/mL), HaCaT (0.625 to 5 μg/mL), and B16- F10 (10 to 40 μg/mL) using MTT assay. BrdU assay showed increased cellular proliferation by 343.23%, 316.13%, and 512.75% at 17.5 μg/mL in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BTDMEM + BT-Test formulation groups, respectively. An increased cellular proliferation by 287.1%, 264.4%, and 428.9% was found in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BTTest formulation groups, respectively at 17.5 μg/mL compared to the UT-DMEM + UT-Test formulation group. Collagen level was increased by 15.70% and 13.52% at 2.5 and 0.625 μg/mL respectively, in the UT-DMEM + BT- Test formulation group, with respect to the UT-DMEM + UT-Test formulation group. The level of elastin was significantly increased by 47.0%, 64.1%, and 11.1% in the BT-DMEM + UT- Test formulation group at 2.5, 1.25, and 0.625 μg/mL, respectively compared to the UT-DMEM + UT-Test formulation group. The level of HA was significantly increased by 4.37%, 41.47%, and 19.74% in the UT-DMEM + BT-Test formulation group, at concentration 2.5, 1.25, and 0.625 μg/mL, respectively with respect to the UT-DMEM + UT-Test formulation group. Melanin levels were reduced by 8.83%, 5.78%, and 12.68% at 0.0125, 0.0625, and 0.125 μg/mL, respectively in the UT-DMEM + BT-Test formulation groups, while melanin was also decreased by 4.73% and 13.21% at 0.0125 and 0.125 μg/mL respectively, in the BT-DMEM + UT-Test formulation group compared with the UT-DMEM + UT-Test formulation group. Antiwrinkling potential with respect to UV-B showed an increased cell viability in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups compared to the UT-DMEM + UT-Test formulation group. Wound healing scratch assay showed a significant migration of fibroblast and keratinocyte cells with an increased percentage of covered area up to 6% in HFF-1 cells, while significant change was also reported in HaCaT cells after treatment with Biofield Energy (The Trivedi Effect®) based test formulation. Overall, the Biofield Energy Treated Test formulation and DMEM can be used significantly in different pharmacological actions such as anti-wrinkling, anti-aging, skin whitening, and wound healing agent. Overall, the Biofield Energy Treated test formulation can be used as a Complementary and Alternative Medicine (CAM) treatment with a safe therapeutic index for various skin irregularities that are typically symptoms of a skin disorders such as Eczema, diaper rash, chickenpox, measles, warts, acne, hives, wrinkles, ringworm, Rosacea, psoriasis, seborrheic dermatitis, skin cancer, rashes from bacterial or fungal infections, rashes from allergic reactions, raised bumps that are red or white, cracked skin, discolored patches of skin, fleshy bumps, warts, or other skin growths, changes in mole color or size, a loss of skin pigment, scaly or rough skin, peeling skin, ulcers, open sores or lesions, dry, excessive flushing. Further, the Biofield Energy Healing Treatment can also be used in the prevention of temporary and permanent skin disorders, anti-aging, improved overall health, and quality of life.