Isotopic Abundance Ratio of Magnesium Gluconate Using LC-MS and NMR Spectroscopy

What is it about?

The objective of the current study was to investigate the effect of The Trivedi Effect® - Energy of Consciousness Healing Treatment (Biofield Energy Healing Treatment) on magnesium gluconate for the change in the structural properties and isotopic abundance ratio (PM+1/PM) using LC-MS and NMR spectroscopy.

Featured Image

Why is it important?

The current research work envisaged the structural characterization of magnesium gluconate using LC-MS and NMR techniques and a significant influence of The Trivedi Effect® – Energy of Consciousness Healing Treatment (Biofield Energy Healing Treatment) on the isotopic abundance ratio of PM+1/PM. The LC-MS analysis of the both control and treated samples revealed the presence of the mass of the protonated magnesium gluconate at m/z 415 at the retention time of 1.53 minutes with the nearly same type of fragmentation. The relative peak intensities of the fragment ions of The Trivedi Effect® Treated sample were significantly changed compared with the control sample. The isotopic abundance ratio of PM+1/PM (2H/1H or 13C/12C or 17O/16O or 25Mg/24Mg) was significantly increased in the treated sample by 80.38% compared with the control sample. Briefly, 13C, 2 H, 17O, and 25Mg contributions from (C12H23MgO14)+ to m/z 416 in the treated sample was significantly increased compared to the control sample. The Trivedi Effect® treated sample might exhibit isotope effects such as altered physicochemical and thermal properties, rate of the reaction, selectivity and binding energy due to its reduced isotopic abundance ratios of PM+1/PM and PM+2/PM compared with the control sample. The Trivedi Effect® treated magnesium gluconate might be helpful to understand the enzymatic reactions as well as design the novel potent enzyme inhibitors by using its kinetic isotope effects. Besides, The Trivedi Effect®- Energy of Consciousness Healing Treatment (Biofield Energy Healing Treatment) could be a useful approach in the design of better nutraceutical and/or pharmaceutical formulations that can offer significant therapeutic responses against various diseases such as diabetes mellitus, allergies and septic shock, stress-related disorders like sleep disorder, insomnia, anxiety, depression, Attention Deficit Disorder (ADD), Attention Deficit Hyperactive Disorder (ADHD), mental restlessness (mind chattering), brain frog, low libido, impotency, lack of motivation, mood swings, fear of the future, confusion, migraines, headaches, forgetfulness, overwhelm, loneliness, worthlessness, indecisiveness, frustration, irritability, chronic fatigue, obsessive/compulsive behavior and panic attacks, inflammatory diseases and immunological disorders like Lupus, Systemic Lupus Erythematosus, Hashimoto Thyroiditis, Type 1 Diabetes, Asthma, Chronic peptic ulcers, Tuberculosis, Hepatitis, Chronic active hepatitis, Celiac Disease (gluten-sensitive enteropathy), Addison Disease, Crohn’s disease, Graves’ Disease, Pernicious and Aplastic Anemia, Sjogren Syndrome, Irritable Bowel Syndrome (IBS), Multiple Sclerosis, Rheumatoid arthritis, Chronic periodontitis, Ulcerative colitis, Chronic sinusitis, Myasthenia Gravis, Atherosclerosis, Vasculitis, Dermatitis, Diverticulitis, Rheumatoid Arthritis, Reactive Arthritis, Alopecia Areata, Psoriasis, Scleroderma, Fibromyalgia, Chronic Fatigue Syndrome and Vitiligo, aging-related diseases like cardiovascular disease, arthritis, cancer, Alzheimer’s disease, dementia, cataracts, osteoporosis, diabetes, hypertension, glaucoma, hearing loss, Parkinson’s Disease, Huntington’s Disease, Prion Disease, Motor Neurone Disease, Spinocerebellar Ataxia, Spinal muscular atrophy, Amyotrophic lateral sclerosis, Friedreich’s Ataxia and Lewy Body Disease; chronic infections and many more.