Herbomineral Formulation on Pro-inflammatory Cytokines Expression in Mouse Splenocytes
What is it about?
The objective of the study was to evaluate the in vitro effect of Biofield Energy Healing (The Trivedi Effect®) on the test formulation using murine splenocyte cells.
Why is it important?
Based on the obtained results, it was concluded that the Biofield Energy Treated test formulation modulates the splenocyte cells function with respect to the pro-inflammatory cytokines TNF-α, IFN-γ, MIP-1α, and IL-1β. However, the Biofield Treated test formulation significantly inhibited the activity of pro-inflammatory cytokines with respect to the untreated test formulation. In vitro cells viability assay showed that all the tested concentrations of the herbomineral formulation were found safe with respect to the vehicle control group. The percentage cell viability ranged from 88.9% to 187.2% in different concentration ranges of the test formulation, so concentration ranges from 0.00001053 to 10.53 µg/mL of the test formulation were selected for the splenocyte cells for the cytokines estimation. TNF-α levels were significantly inhibited at 0.001053 and 0.1053 µg/mL, in which the Biofield Treated test formulation group showed suppression by 1.70% and 8.16% as compared with the untreated test formulation. Similarly, significant suppression of IFN-γ expression was reported at five concentrations i.e. at 0.00001053, 0.0001053, 0.01053, 0.1053, and 1.053 µg/mL of the Biofield Treated test formulation by 12.63%, 2.31%, 8.31%, 9.15%, and 7.86%, respectively as compared with the untreated test formulation. This might help in inflammatory tissue response and can be used in many inflammatory disorders. In the case of MIP-1α secretion, the expression was inhibited by the Biofield Treated test formulation at three tested concentrations i.e. at 0.0001053, 0.01053, and 0.1053 µg/mL by 8.31%, 21.53%, and 8.70%, respectively as compared with the untreated test formulation. IL-1β secretion was significantly suppressed only at a very low concentration i.e. 0.00001053 µg/mL by 19.72% as compared with the untreated test formulation. On the basis of the experimental results of the various tested cytokines showed significant immunosuppressive activity in the new herbomineral formulation after treatment with The Trivedi Effect®– Biofield Energy Healing (TEBEH) by the group of twenty renowned Biofield Energy Healers. Overall, The Trivedi Effect®– Biofield Energy Healing Treated test formulation significantly inhibited the activity of pro-inflammatory cytokines and showed immunosuppressive activity with a safe therapeutic index as compared with the untreated test formulation. Biofield Energy Healing shows great promise as a complementary and alternative medicine therapy and might play an important role in autoimmune and inflammatory diseases like Chronic peptic ulcers, Tuberculosis, Hepatitis, Celiac Disease (gluten-sensitive enteropathy), Addison Disease, Irritable Bowel Syndrome, Multiple Sclerosis (MS), Parkinson’s Disease, Graves’ Disease, Rheumatoid arthritis, Chronic periodontitis, Ulcerative colitis, Crohn’s disease, Chronic sinusitis, Lupus, Systemic Lupus Erythematosus, Hashimoto Thyroiditis Type 1 Diabetes, Asthma, Chronic active hepatitis, Myasthenia Gravis, Rheumatoid Arthritis, Reactive Arthritis, Pernicious and Aplastic Anemia, Sjogren Syndrome, Alopecia Areata, Psoriasis, Scleroderma, Fibromyalgia, Chronic Fatigue Syndrome, Vitiligo, Vasculitis, Alzheimer’s Disease, Atherosclerosis, Dermatitis, Diverticulitis, stress, etc. Biofield Energy Healing may also be useful in the prevention of immune-mediated tissue damage in cases of organ transplants (for example heart transplants, kidney transplants and liver transplants), stress prevention and management, for anti-aging and in improving overall health and quality of life.
The following have contributed to this page: Mr Mahendra Kumar Trivedi, Ariadne Afaganis, Daphne Luisa Dumas, Denise Fiedler, Desi Pano, Donna Alija, Brian A. Weekes, Haddon Salt, Inthirani Arul, James Burnett, Jean White, Dennille Smith, Dahryn Trivedi, Alice Branton, and Gopal Nayak