Designing of nematode egg parasitic fungus, Purpureocillium lilacinum, nanoemulsion for management of Meloidogyne incognita infection in tomato

What is it about?

A new nano-formulation of Purpureocillium lilacinum (P. lilacinum) targets Meloidogyne spp. and other nematodes in crops, addressing issues of short shelf life and inconsistent performance of existing commercial formulations. Developed and tested against Meloidogyne incognita in tomatoes, the formulation underwent rigorous screening, with NE 8 emerging as optimal. NE 8, comprising specific components, exhibited superior characteristics, including a particle size of 226.6 nm, zeta potential of -54.80 mV, and spherical morphology. In vitro tests showed complete immobility of M. incognita juveniles (J2) and egg parasitism at 5.0 mL/L. Glasshouse trials demonstrated enhanced tomato growth and significant nematode suppression, with increased egg mass parasitism, using concentrations from 0.5 to 5.0 mL/L. Bio-safety tests support its application at specified concentrations.

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