Effects of P70 S6 kinase on the ageing process and reproduction of Daphnia pulex (Branchiopoda, Anomopoda)

What is it about?

To explore the relationship between S6K and aging in Daphnia pulex, in this study, To investigate these questions, we first cloned and analysed the full‐length cDNA of S6k and detected S6K mRNA and protein expression in different growth stages of D. pulex. Based on the sequence of S6k, we constructed a vector comprising S6k dsRNA for RNAi. After RNAi knockdown of S6k, we analysed S6K expression and determined the changes in survival rate and reproduction. Bioinformatics was then used to predict miRNAs that control S6k expression, which were verified using dual luciferase reporter assays.

Featured Image