Fiber and Transcriptional Modifications Enhance Adenovirus Targeting towards Human Adhesion Cells

What is it about?

In this study, we screened various transduction and transcription modifications of adenovirus (Ad) to identify those that support maximal Ad-mediated gene delivery to human adhesion fibroblasts, which in turn would enhance the efficacy of this novel treatment/preventative strategy for postoperative adhesions. We found that among the fiber-modified Ad vectors, Ad5-Sigma-luc and among the transcriptional targeting modified Ad vectors, Ad5-MSLN-CRAd-luc showed significantly increased expression levels of luciferase activity in adhesion fibroblast cells compared with wild-type Ad5-luc (p < 0.05). These specific modifications of Ad improve their gene delivery efficiency towards human peritoneal adhesion fibroblasts. Developing a safe localized method to prevent/treat postoperative adhesion formation would have a major impact on health of patients with postoperative abdominal adhesions.

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Why is it important?

Developing a safe localized method to prevent/treat postoperative adhesion formation would have a major impact on health of patients with postoperative abdominal adhesions.