Detection by Flow Cytometry of Anti-DNA Autoantibodies and Circulating DNA Immune Complexes in Lupus Erythematosus

  • Nisen Abuaf, Chantal Desgruelles, Mohamed Moumaris, Faïza Boussa-Khettab, Hidayeth Rostane, Emilie Bellec, Camille Frances
  • Journal of Immunology Research, December 2019, Hindawi Publishing Corporation
  • DOI: 10.1155/2019/6047085

Detection by Flow Cytometry of anti- DNA autoantibodies and immune complexes

What is it about?

A new method for the detection by flow cytometry of anti- DNA antibodies and of circulating immune complexes containing endogenous DNA is described.

Why is it important?

Autoantibodies to double-stranded DNA are the serological hallmark of systemic lupus erythematosus (SLE). When anti-DNA antibodies are neutralized by endogenous DNA and can no longer be detected by routine tests, the serologic diagnosis and the follow-up of relapses in patients with SLE is compromised. To overcome this obstacle, we propose an accessible solution: the detection of circulating DNA immune complexes by flow cytometry.

Perspectives

Nisen ABUAF
Faculté de Médecine, Sorbonne Université

The results of routine tests for the detection of anti-DNA autoantibodies do not correlate well. This could be due to the fact that lupus serum samples contain both anti-DNA autoantibodies, endogenous DNA and immune complexes. In the near future the tests should be designed and the results interpreted by integrating this data.

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http://dx.doi.org/10.1155/2019/6047085

The following have contributed to this page: Dr. Mohamed Moumaris and Nisen ABUAF