What is it about?
Voltage-gated proton channel (Hv1) has been studying in various immune cells, but most studies have relied on in vitro approach. Here, we have established the in vivo system for functional analysis of Hv1 using zebrafish as the animal model. We further examined the real-time Hv1-related behavior in neutrophils during tissue injury and acute bacterial infection.
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Why is it important?
We generated the Hv1-deficient zebrafish and confirmed that the key properties of Hv1 are conserved in zebrafish neutrophils. We further examined the behavior of neutrophils using our zebrafish model, providing the first evidence for real-time observation of Hv1 function inside the living animal. The model system also allowed us to detect a potential link between Hv1 and phagosome dynamics in neutrophils during active phagocytosis. These findings indicate that zebrafish is a useful model for investigating the Hv1 function in a physiological context that could be applied for future research in this field.
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This page is a summary of: Real-time functional analysis of Hv1 channel in neutrophils: a new approach from zebrafish model, AJP Regulatory Integrative and Comparative Physiology, June 2019, American Physiological Society,
DOI: 10.1152/ajpregu.00326.2018.
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