What is it about?
Dermatophytoses (ringworm, jock itch, athlete’s foot and nail infections) are the most common fungal infections, but their virulence mechanisms are poorly understood. Combining transcriptomic data obtained from growth in various culture conditions with data obtained during infection led to a significantly improved genome annotation. About 65% of the protein-coding genes predicted with our protocol did not match the existing annotation for A. benhamiae. Comparing gene expression during infection on guinea pigs versus keratin degradation in vitro, which is supposed to mimic the host environment, revealed the critical importance of using real in vivo conditions for investigating virulence mechanisms.
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Why is it important?
Comparing gene expression during infection phase versus keratin degradation in vitro shows the importance of using real in vivo conditions to further investigate the virulence mechanisms of dermatophytes, instead of using some in vitro conditions supposed to mimic the host environment.
Perspectives
The analysis of genes expressed in vivo, encoding cell surface and secreted proteins, particularly proteases, led to the identification of new allergen and virulence factor candidates.
Prof. Michel Monod
Access Careers
Read the Original
This page is a summary of: RNA Sequencing-Based Genome Reannotation of the Dermatophyte Arthroderma benhamiae and Characterization of Its Secretome and Whole Gene Expression Profile during Infection, mSystems, August 2016, ASM Journals,
DOI: 10.1128/msystems.00036-16.
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