What is it about?
We compared the performance of VLP- and NS1-MAC-ELISA from both JEV and WNV and both assays showed high sensitivity but slightly lower specificity, depending on what type of virus infection for diagnosis. However, combining the use of multiantigen VLP- and NS1-MAC-ELISAs significantly increase both the sensitivity and specificity.
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Why is it important?
Due to antibody cross-reactivity among different flaviviruses, development of serological assay capable of differentiating antibodies from different flavivirus infection is crucial for diagnosis, sero-survey and evaluation of vaccine effectiveness. Traditionally, detecting anti-E antibodies is a favorable serological assay in diagnosing flavivirus infection. However, recent studies suggest that anti-NS1 antibodies could be specific than anti-E antibodies. Combining both assays of VLP- and NS1-MAC-ELISA could significantly enhance our diagnostic capability and deepen the understanding of flavivirus serological assay.
Perspectives
In the era of post-Zika outbreak in American and DengVaxia, NS1-MAC(GAC)-ELISA and in combination with VLP-MAC(GAC)-ELISA can significantly enhance the diagnostic capability of flavivirus infection.
Dr Day-yu Chao
national chung-hsing university
Read the Original
This page is a summary of: Establishment of an Algorithm Using prM/E- and NS1-Specific IgM Antibody-Capture Enzyme-Linked Immunosorbent Assays in Diagnosis of Japanese Encephalitis Virus and West Nile Virus Infections in Humans, Journal of Clinical Microbiology, December 2015, ASM Journals,
DOI: 10.1128/jcm.02469-15.
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