Culture method for isolating Campylobacter species from stool samples

What is it about?

We used a filtration method for isolating Campylobacter species (not C jejuni or C coli) from stool samples using a microaerobic environment with increased hydrogen concentration. We showed that Campylobacter species could be isolated from stools using this method and some isolates appeared to be clinically relevant.

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Why is it important?

Previous studies, mostly from European clinical laboratories had shown that filtration was a useful method for isolating species other than C jejuni/ C coli from human stool samples, however, such a study had not been reported from a US clinical laboratory. The study confirmed recent publications showing that polycarbonate filters were superior to nitrocellulose filters and that increased hydrogen was essential for isolating unusual Campylobacter species.