What is it about?
The phylogeny of Shiga toxin-producing Escherichia coli strains has been extensively investigated; however, links in their relatedness to potentially pathogenic Shiga toxin-negative, eae-positive E. coli O26:H11 strains remain missing. This work shows that Shiga toxin-negative, eae-positive E. coli O26:H11 strains have a close phylogenetic relationship with pathogenic Shiga toxin-positive human clinical strains. Using a high-throughput library preparation technique for next-generation sequencing of targeted single-nucleotide polymorphisms, this work demonstrates an expedited strain classification and characterization method.
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This page is a summary of: Targeted Amplicon Sequencing for Single-Nucleotide-Polymorphism Genotyping of Attaching and Effacing Escherichia coli O26:H11 Cattle Strains via a High-Throughput Library Preparation Technique, Applied and Environmental Microbiology, November 2015, ASM Journals,
DOI: 10.1128/aem.03182-15.
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