Genomic Diversity of Epstein-Barr Virus Genomes Isolated from Primary Nasopharyngeal Carcinoma Biopsy Samples

H. Kwok, C. W. Wu, A. L. Palser, P. Kellam, P. C. Sham, D. L. W. Kwong, A. K. S. Chiang
  • Journal of Virology, July 2014, ASM Journals
  • DOI: 10.1128/jvi.01665-14

EBV genomes from primary NPC biopsies

What is it about?

This study established a workflow from viral DNA capture and sequencing to de-novo assembly and contig joining and reported eight new EBV genomes isolated from NPC biopsy specimens and revealed diversity on a whole-genome scale among the EBV isolates.

Why is it important?

At least two lineages of EBV strains are revealed. This work provides a platform for genomic sequencing of EBV on a larger scale and may enable a systematic investigation on the role of variations/mutations in the viral genome in the pathogenesis of EBV-associated cancers such as nasopharyngeal carcinoma.

Perspectives

Dr Alan KS CHIANG
The University of Hong Kong

This work describes the workflow of enrichment and next generation sequencing (NGS) of EBV genomes in clinical biopsy specimens and the de-novo assembly of the viral genomes. This provides the platform and impetus for large scale NGS of EBV genomes harbored in diseased tissues of patients and in saliva or blood of healthy controls.

Read Publication

http://dx.doi.org/10.1128/jvi.01665-14

The following have contributed to this page: Dr Alan KS CHIANG