Performance of the 47-Kilodalton Membrane Protein versus DNA Polymerase I Genes for Detection of Treponema pallidum by PCR in Ulcers

Angèle Gayet-Ageron, Frédéric Laurent, Jacques Schrenzel, Béatrice Charton, Gisela Jimenez-Getaz, Manuela Tangomo, Tristan Ferry, Patrice Sednaoui, Stephan Lautenschlager, Laurence Toutous-Trellu, Begoña Martinez de Tejada, Matthias Cavassini, Stéphane Emonet, Thomas Perneger, Hélène Salord
  • Journal of Clinical Microbiology, December 2014, ASM Journals
  • DOI: 10.1128/jcm.03444-14

What is it about?

This study has compared the diagnostic performance of the two targets currently used to amplify Treponema pallidum by PCR: 47kDa protein membrane versus polymerase I gene. We demonstrated that the two targets provide similar accuracy and could be univocally used in routine.

Why is it important?

This is the first study providing a comparison of the diagnostic performance of the two PCRs used in the diagnosis of syphilis on the same samples. This comparison was done on paired samples from a large collection of swabs (n=272). The findings of this study showed the two targets provide the same diagnostic performance and have an almost perfect agreements.

The following have contributed to this page: Dr Angèle Gayet-Ageron