Malic Acid Production by Saccharomyces cerevisiae: Engineering of Pyruvate Carboxylation, Oxaloacetate Reduction, and Malate Export

  • Rintze M. Zelle, Erik de Hulster, Wouter A. van Winden, Pieter de Waard, Cor Dijkema, Aaron A. Winkler, Jan-Maarten A. Geertman, Johannes P. van Dijken, Jack T. Pronk, Antonius J. A. van Maris
  • Applied and Environmental Microbiology, March 2008, ASM Journals
  • DOI: 10.1128/aem.02591-07

What is it about?

Engineering yeast to make malic acid from glucose

Why is it important?

Engineering of yeast for the production of C4-dicarboxylic acids (malate, succinate) is of great industrial interest. This paper is, to my knowledge, the first demonstration that successful engineering of yeast for production of these compounds requires the combined overexpression of a carboxylating enzyme, an oxidoreductase and, importantly, an exporter.


Prof Jack T Pronk
Technische Universiteit Delft

This study may be (one of the) first demonstration(s) of the successful engineering of product export in yeast - please correct me if I'm wrong! This research was the result of a very stimulating long-term collaboration with Tate & Lyle on organic acid production by engineered yeast, which not only taught me a lot about yeast, but also on how to make collaborations between industry and academia work. Thanks Jeff Lievense, Stan Bower and Chi-Li Liu, who were key partners at the industrial side of the collaboration!

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The following have contributed to this page: Dr Rintze M Zelle and Prof Jack T Pronk

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