Semirational Directed Evolution of Loop Regions in Aspergillus japonicus β-Fructofuranosidase for Improved Fructooligosaccharide Production

K. M. Trollope, J. F. Görgens, H. Volschenk
  • Applied and Environmental Microbiology, August 2015, ASM Journals
  • DOI: 10.1128/aem.02134-15

An improved β-fructofuranosidase for industrial sucrose-to-fructooligosaccharide biotransformation

What is it about?

Fructooligosaccharides are produced from sucrose using an Aspergillus japonicus β-fructofuranosidase. Efficient sucrose biotransformation is however not realized due to the enzyme's limitations under industrial conditions. In this paper we used a semi-rational protein engineering approach, using combined crystal structure and evolutionary-guided data, to engineer loops regions of the enzyme to yield a variant displaying improved specific activity and thermostability able to reduce the time to completion of an industrial-like FOS synthesis reaction by 26%.

Why is it important?

The positive outcome of the semi-rational approach showed that engineering loops regions in an enzyme is a feasible strategy to improve both specific activity and thermostability, most probably due to the modification of enzyme structural flexibility.

The following have contributed to this page: Dr Heinrich Volschenk