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Why is it important?
Our analysis shows that the smaller pixel size and faster readout speed of scientific-grade complementary metal oxide semiconductor (sCMOS) enables SOFI to achieve high spatiotemporal resolution with a large field-of-view, which is especially beneficial for live-cell super-resolution imaging, when fluorescent signal is not extremely low.
Perspectives
As the performance of SOFI is relatively insensitive to the signal-to-noise ratio (SNR), the gain in pixel size and readout speed exceeds the loss in SNR, indicating sCMOS is superior to electron multiplying charge coupled device in context to SOFI in many cases.
Dr Peng Xi
Peking University
Read the Original
This page is a summary of: Superior performance with sCMOS over EMCCD in super-resolution optical fluctuation imaging, Journal of Biomedical Optics, June 2016, SPIE,
DOI: 10.1117/1.jbo.21.6.066007.
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