Sustained activation of proton channels and NADPH oxidase in human eosinophils and murine granulocytes requires PKC but not cPLA2α activity

What is it about?

We test and discard the prevailing hypothesis that proton channels are activated by arachidonic acid (AA) produced by cPLA2a. Specific blockers have no effect, nor is the response changed in knockout mice. However, PKC inhibitors prevent activation of NADPH oxidase and H channels, and can reverse their activation. Most surprisingly, part of the effect of AA is sensitive to PKC inhibitors; hence AA activates PKC, not vice versa!

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