Phospholipases D with C2 domain are involved in different reactions induced by oxygen deficiency

What is it about?

Different types of stress usually induce an increase in calcium, reactive oxygen species (ROS), and phosphatidic acid (PA) levels in plants. To investigate the role of phospholipases D (PLDs) in stress signalling during oxygen deprivation, hypoxia, we used Arabidopsis wild type and 10 isoform mutants with C2 domains. The C2 domains have Ca binding loops and are involved in binding proteins to cell membranes. Here we studied the hypoxia-induced changes of these signalling players in the wild type and mutants and found that the elevation of calcium and PA was reduced in all mutants. compared with wild type. Pharmacological experiments with the wild type confirmed that both external and internal sources contributed to calcium and ROS accumulation under hypoxia. However, some of the mutants were involved in calcium signalling and PA production, while all PLDs investigated, except for one, were responsible for ROS elevation.

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Why is it important?

For the fist time we were able to show that all PLDS with C2 domains and PA are involved in hypoxia signalling in Arabidopsis.

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