What is it about?

A Trypanosoma brucei cell line was generated that exclusively expresses an analog-sensitive version of the cyclin-dependent kinase CRK9 (CRK9AS1) which functions in conjunction with cyclin CYC12 and CRK9-associated protein (CRK9AP). Specific inhibition of this enzyme with a bulky ATP-competing inhibitor demonstrated that the kinase activity of CRK9 was essential for spliced leader trans splicing. The splicing block occurred very rapidly, suggesting that CRK9 carries out reversible phosphorylation on the RNA processing machinery.

Featured Image

Read the Original

This page is a summary of: Rapid block of pre‐mRNA splicing by chemical inhibition of analog‐sensitive CRK9 inTrypanosoma brucei, Molecular Microbiology, March 2020, Wiley,
DOI: 10.1111/mmi.14489.
You can read the full text:

Read

Contributors

The following have contributed to this page