What is it about?
The levels of cytochrome P450 and oxidative activities of cytochrome P450 monooxygenases were enhanced in a fenvalerate-resistant strain (HDFR) of Helicoverpa armigera in China, comparing with those of a susceptible strain (HDS) in our previous studies. In the present study, we report the molecular cloning and gene expression of a specific P450 CYP6B7 from HDFR and HDS strain of H. armigera.
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Why is it important?
There were three substitutions of amino acids in the coding region of CYP6B7 cDNA and three mutative nucleotides in the intron of CYP6B7 genomic DNA of HDFR strain, resepctively, comparing with that of HDS strain. The expression level of CYP6B7 mRNA in HDFR strain was > 5-fold higher than that in HDS strain. Addtionally, the expression level of CYP6B7 mRNA increased as the larvae developed; however, it was rarely detected in the pupae and adult stages. The results suggested that CYP6B7 plays an important role in the fenvalerate resistance of HDFR strain of H. armigera; overexpression of CYP6B7 combined with relevant substitution of amino acids might be responsible for fenvalerate-resistance in H. armigera from China.
Perspectives
In this study, we reported interaction between cytochrome P450 CYP6B7 of Helicoverpa armigera and fenvalerate resistance. Our results suggested that overexpression of CYP6B7 combined with relevant substitution of amino acids might be responsible for fenvalerate-resistance in H. armigera from China.
Ph.D Tao Tang
Institute of Plant Protection, Hunan Academy of Agricultural Sciences
Read the Original
This page is a summary of: Cloning and expression of cytochrome P450 CYP6B7 in fenvalerate-resistant and susceptible Helicoverpa armigera (Hübner) from China, Journal of Applied Entomology, November 2010, Wiley,
DOI: 10.1111/j.1439-0418.2009.01496.x.
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