What is it about?
The coelomic fluid (CF) noticeably decreased the viability and affected the morphology of the A549 cells. Scanning electron microscopy revealed a different degree of destruction of the nucleus and cytoplasm of A549 cells. A twofold increase in the caspase 3, 4, 5, and 10 levels was observed in the A549 cells after the incubation with the CF. The results obtained by flow cytometry using Annexin V confirmed the proapoptotic effect of the earthworm CF on A549 lung cancer cells. The D. veneta CF and active fraction obtained with cytotoxicity toward A549 lung cancer is an interesting and promising preparation for further biological, chemical, and biomedical research.
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Why is it important?
Our study demonstrated that coelomic fluid of the earthworm D. veneta showed anticancer activity against A549 lung cancer cells but did not exhibit cytotoxicity toward the bronchial epithelial cell line BEAS-2B. It was demonstrated that the heated coelomic fluid caused the death of cancer cells via apoptosis. This is the first description of the selective effect of the preparation and the protein fraction obtained from the coelomic fluid on A549 lung cancer cells.
Perspectives
We have obtained a fraction from the earthworm coelomic fluid, which in 90% destroys A549 lung cancer cells, and at the same time does not destroy normal cells.These data may contribute to the development of a new drug for the treatment of non-small cell lung cancer.
Marta Fiolka
Maria Curie-Skłodowska University in Lublin, Poland
Read the Original
This page is a summary of: Antitumor activity and apoptotic action of coelomic fluid from the earthworm
Dendrobaena veneta
against A549 human lung cancer cells, Apmis, March 2019, Wiley,
DOI: 10.1111/apm.12941.
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