What is it about?

High-fat diet (HFD) feeding is most commonly used mice model for investigating the interactions between fat and bone metabolism under obesity states. However, contradictory and confusing information is still available. In this study, we summarized data from 80 articles to determine the alterations in bone mass of femur, tibia, and vertebrae in C57BL/6 mice after HFD. Overall, HFD led to both cortical and trabecular bone loss in femur, tibia and vertebrae in mice. In addition, male mice or mice aged 6–12 weeks old are relatively susceptible to HFD. HFD with > 50% of energy from fats and intervention time of 10 weeks to 5 months are more likely to induce skeletal alterations. These findings supported HFD as an appropriate model for obesity-associated bone loss and can guide future studies.

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Why is it important?

This is the first systematic review and meta-analysis to explore the effects of HFD on the bone metabolism of mice. We drawed some interesting conclusions and deduces which would inspire further studies.


The prevelance of obesity and bone diseases is increasing rapidly. More and more evidence showed obesity, especially severe obesity, exerts negative impact on bone health. Researchers are using high-fat diet in mice to invesitigate the interactions between fat and bone metabolism under obesity states, along with the underlying cellular and molecular mechanisms.

Zheng Zhang

Read the Original

This page is a summary of: How high‐fat diet affects bone in mice: A systematic review and meta‐analysis, Obesity Reviews, July 2022, Wiley,
DOI: 10.1111/obr.13493.
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