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The Enterococcal conjugative transposon Tn1549 spreads vancomycin resistance between bacteria. Here, we show efficient and accurate movement of a mini-Tn1549 version in E. coli and determine that jumping from donor to target DNA site requires specific DNA sequences in the transposon and two transposon proteins XIS and INT. When XIS is absent or mutations occur in the DNA, the transposon transfers additional DNA with it; this rescues transposon movement and mediates transfer of extra DNA.
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This page is a summary of: Conjugative transposition of the vancomycin resistance carrying Tn1549: enzymatic requirements and target site preferences, Molecular Microbiology, January 2018, Wiley,
DOI: 10.1111/mmi.13905.
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