Mechanism of increased microbial autofluorescence by heat treatment

T. Tominaga, K. Fujioka, M. Hijikuro, T. Tada, Y. Nishikawa
  • Letters in Applied Microbiology, January 2018, Wiley
  • DOI: 10.1111/lam.12828

Heat treatment makes microbes fluoresce via the Maillard reaction

What is it about?

We had looked for an easy method to make microbes microscopically countable. We noted microbes fluoresce after the heat treatment without any staining; the Maillard reaction produced advanced glycation end products (AGEs) and resulted in the enhanced autofluorescent signals.

Why is it important?

The easy method to make microbes microscopically countable was vital for our developing an automatic sensor that can count microbes microscopically. Based on this findings we could manufacture the compact device to enumerate microbes floating in ambients (https://www.slsjp.co.jp/microbe-sensor).

Perspectives

Professor Yoshikazu Nishikawa (Author)
Osaka City University

We should use the apparatus in fields to show how well the data correlate with those collected by conventional culture methods.

The following have contributed to this page: Professor Yoshikazu Nishikawa