Skin swabbing as a new efficient DNA sampling technique in amphibians, and 14 new microsatellite markers in the alpine newt (Ichthyosaura alpestris)

J. PRUNIER, B. KAUFMANN, O. GROLET, D. PICARD, F. POMPANON, P. JOLY
  • Molecular Ecology Resources, January 2012, Wiley
  • DOI: 10.1111/j.1755-0998.2012.03116.x

Skin swabs and buccal swabs in amphibians.

What is it about?

The sampling of DNA in threatened species such as amphibians has to be the less invasive as possible. Here, we showed that the swabbing of skin provided DNA in reasonable quantity and quality, and allowed accurate individual genotyping while limiting the handling of animals.

Why is it important?

Skin swabbing provided roughly as much DNA as the more traditional buccal swabbing, with similar genotyping succes, but without the inconvenients of buccal swabs (bleeding, etc.)

Perspectives

Dr Jérôme G. Prunier
Station d'Ecologie Expérimentale du CNRS à Moulis

Skin swabs, already considered for chytrid sampling, is proposed as a new standard for genetic sampling in amphibians, especially threatend ones.

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http://dx.doi.org/10.1111/j.1755-0998.2012.03116.x

The following have contributed to this page: Dr Jérôme G. Prunier