Characterization of beta-amyrin synthase from Euphorbia tirucalli
What is it about?
Expression of beta-amyrin synthase in Saccharomyces cerevisiae lacking lanosterol synthase. Km and kcat values are reported. Ki values of a few inhibitors are determined. Proton donor to initiate the polycyclization reaction is determined by the site-directed mutagenesis.
Why is it important?
Complete purification of oxidosqualene cyclases has not been reported except for human lanosterol synthase. This is the second paper for the complete purification. Thus, we could report the detailed enzymatic characterization of beta-amyrin synthase.
The following have contributed to this page: Tsutomu Hoshino