What is it about?
With Recentrifuge, researchers can interactively explore what organisms are in their samples and at which level of confidence (score), thus enabling robust comparative analysis of multiple samples in any metagenomic study. In addition to the scored charts for the original specimens, Recentrifuge generates scored plots for shared taxa and exclusive taxa per sample. If there are one or more negative control samples in the study, Recentrifuge will also generate control-subtracted charts and a shared taxa plot with the controls removed. The robust control removal algorithm detects and selectively extracts various types of contamination, including crossovers. Recentrifuge is free software available on GitHub. For more information, please visit www.recentrifuge.org.
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Why is it important?
Recentrifuge is especially useful in the case of low microbial biomass metagenomic studies and when a more reliable detection of minority organisms is needed, like in clinical, environmental and forensic analysis.
Perspectives
Recentrifuge currently supports Centrifuge and LMAT taxonomic classifiers, but there are active plans to support CLARK and Kraken.
Jose Manuel Martí
University of California Berkeley LBNL
Read the Original
This page is a summary of: Recentrifuge: robust comparative analysis and contamination removal for metagenomic data, September 2017, Cold Spring Harbor Laboratory Press,
DOI: 10.1101/190934.
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