Comparison and Differentiation of Potyvirus Isolates and Identification of Strain-, Virus-, Subgroup-specific and Potyvirus Group-common Epitopes Using Monoclonal Antibodies

R. Jordan, J. Hammond
  • Journal of General Virology, January 1991, Society for General Microbiology
  • DOI: 10.1099/0022-1317-72-1-25

Monoclonal antibodies for detection and differentiation of potyviruses

What is it about?

Creation and utilization of monoclonal antibodies to detect and differentiate potyviruses. A panel of monoclonal antibodies raised against a mixture of various potyviruses yielded a diverse spectrum of reactivities, from highly cross-reactive, to virus-specific, to one antibody specific for a particular strain of bean yellow mosaic virus

Why is it important?

These antibodies have utility for the sensitive detection of different potyviruses, and are valuable for the rapid screening of plants to determine whether there is a potyvirus infection present. The antibody with broadest cross-reactivity has been used worldwide for viral diagnostics in a variety of formats.

Perspectives

John Hammond
USDA-ARS, USNA, FNPRU

This was the first demonstration of an essentially pan-reactive monoclonal antibody that could be used to detect potyviruses; previously available polyclonal antisera were either highly virus-specific, or recognized only a limited number of different potyviruses. In addition to the pan-reactive antibody, other monoclonal antibodies with varying degrees of specificity have other applications, including determining that transgenically expressed potyviral coat protein could be incorporated into the virions of distinct potyviruses able to infect the transgenic plants.

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http://dx.doi.org/10.1099/0022-1317-72-1-25

The following have contributed to this page: John Hammond