A Simple Dynamic Strategy to Deliver Stem Cells to Decellularized Nerve Allografts

What is it about?

Successful repair of segmental peripheral nerve defects remains a clinical challenge. While processed nerve allografts have been used both experimentally and clinically, in large nerve defects, functional outcomes are not comparable to autograft reconstruction yet. The addition of Mesenchymal Stromal Cells (MSCs) to decellularized nerve allografts may improve outcomes of nerve regeneration. Prior techniques used for cell seeding are traumatic to both the MSCs and nerve graft, and differences in seeding strategies have varying outcomes. In this study, we present an adequate, reliable and validated cell seeding technique that is suitable for stem cell seeding of acellular nerve grafts, but can also be applied to seed other grafts or tubular structures.

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